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技术规格
Background:
Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
Applications:
ELISA, WB, IHC
Name of antibody:
RPA2
Immunogen:
Full length fusion protein
Full name:
replication protein A2, 32kDa
Synonyms:
REPA2; RPA32; RP-A p32; RP-A p34
SwissProt:
P15927
ELISA Recommended dilution:
2000-5000
IHC positive control:
Human breast cancer
IHC Recommend dilution:
30-150
WB Predicted band size:
29 kDa
WB Positive control:
Hela, huvec, HepG2, A549, Jurkat, 231 and A431 cells
WB Recommended dilution:
500-2000